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A deep understanding of the cattle gastrointestinal microbiome is crucial to selective breeding high-efficiency animals that produce more and generate less environmental damage. Here we performed the taxonomic identification of Bacterial and Archaeal communities using high throughput 16SrRNA gene sequencing from critical compartments of the gastrointestinal tract of Bradford cattle raised in a natural grassland in the Pampa biome, Brazil. We analyzed 110 samples, including saliva, ruminal fluid, and feces from 36 months old Bradford heifers (weighing on average 343 ± 30 kg by the sampling time). To reduce unexpected variation and confounders, we selected the animals from the same breed, submitted them to the same food source, and collected the samples for three consecutive years from different animals in the same season. Our main goal was to analyze the microbial shifts throughout the gastrointestinal tract to reference future works proposing management strategies and interventions to improve animal nutrition and increase production in the Pampa Biome. To accomplish our objective, we accessed the microbial community differences in groups with a high and low weight gain controlling for food ingestion and quality of grazed pasture. Few taxa were shared among the samples. About 40% of the phyla and 60% of the genera were unique from saliva samples, and 12.4% of the microbial genera were uniquely found in feces. All samples shared only 36.1% of phyla and 7.5% of genera. Differences in microbial diversity and taxa counts were observed. The ruminal fluid presented the lowest microbial richness, while saliva and feces presented the highest microbial richness. On the other hand, saliva and feces also presented more distinct communities between themselves when compared with ruminal samples. Our data showed that the saliva microbiome is not representative of the rumen microbiome and should not be used as an easy-to-collect sample for studies about the rumen microbiome.
Citation: de Freitas AS, Gan FC, de David DB, Wurdig Roesch LF (2022) The microbiome shifts throughout the gastrointestinal tract of Bradford cattle in the Pampa biome. PLoS ONE 17(12): e0279386.
The Brazilian Pampa is a grassland-dominated environment characterized by an intense agriculture activity based on livestock in association with crop rotation using rice, soybeans, corn, and wheat [3]. These natural grasslands are dominated by about 450 gramineous species and nearly 200 leguminous species. Such characteristics of the Biome give it a natural aptitude for raising ruminants, an activity introduced in the 17th century. Nevertheless, the natural grasslands from the Brazilian Pampa present high seasonality of forage production and nutritional quality throughout the year. Management strategies aiming the rumen development and the establishment of rumen microbiota and interventions to improve animal nutrition should be implemented to increase production. Interventions into the cattle microbiome might be one of those strategies. For instance, using the probiotic bacteria Bacillus subtilis natto benefits the development of cellulolytic microbes in the gastrointestinal tract of calves [4]. Members of the genus Coprococcus have been associated with an efficient rumen microbiome capable of exhibiting increased propionate and butyrate production [5]. A stable microbial load of Lactobacillus species has been shown to improve weight gain and immunocompetence in young calves [6]. Still, a deeper understanding of the microbiome shifts along the gastrointestinal tract is necessary to take full advantage of these strategies.
Here we performed the taxonomic identification of Bacterial and Archaeal communities from critical compartments of the gastrointestinal tract of grass feed Bradford cattle raised in natural grassland in south Brazil. We hypothesized that different gastrointestinal compartments (saliva, ruminal fluid, and feces) harbor unique microbial communities. To test this hypothesis, we analyzed 110 samples from animals from the same breed, submitted them to the same food source, and collected them during the same season. Our main target was to characterize the microbiome shifts along the gastrointestinal tract to support future works aiming at generating interventions to improve rumen development, the establishment of a healthy rumen microbiota, and maintaining and improving animal nutrition.
The main genera enhanced in saliva were Moraxella, Porphyromonas, and Bibersteinia, all of them gram-negative and oxidase-positive bacteria members of the regular oral cavity of animals [67, 68]. Those genera are aerotolerant anaerobes. They can tolerate, for a limited time, the presence of atmospheric oxygen, growing in the presence of oxygen concentrations in the mouth, allowing them to survive in saliva samples. On the other hand, in the rumen, there is an enrichment of bacterial genera which degrade complex carbohydrates, such as Fibrobacter and Prevotellaceae members [69, 70]. The pattern of anaerobic bacteria is also present in fecal samples, but with the increased abundance of Bacteroides, Alistipes, and Ruminococcaceae members, which are commonly found in animal and human feces but have less impact in the degradation of complex carbohydrates once these molecules are mainly degraded in previous compartments of cattle GIT [71].
Another hill torrent from Kala Baga Khosar releasing a stream of 15,000 cusecs floodwater, while Nullah Suri South carrying another 4,000 cusecs water flow, Nullah Suri North 2,100 cusecs and Patok torrent with 900 cusecs of floodwater, according to the Flood Control Room.
According to officials hill torrents originated from Suleman Mountain Range following recent heavy spell of monsoon rains were the ferocious most and affecting over 100,000 people besides damaging homes and crops in Dera Ghazi Khan and Rajanpur, the two districts sandwitched between hill torrents from mountains in the west and river Indus in the east.
According to reports, the water from Indus River did not touch the alarming levels as it did in 2010 floods, the hill torrents from Koh-e-Suleman did the most damage this year, leaving behind a trail of homelessness and material losses like livestock, crops and valuables in homes.
Moreover, Zangi nullah also carrying 2,700 cusecs of flood stream, officials said. The hill torrents raging with heavy flood flow could inflict heavy losses to the nearby settlements, officials have apprehended.
According to officials, hill torrents originated from Suleman Mountain Range following recent heavy spell of monsoon rains were the ferocious most and affecting over 100,000 people besides damaging homes and crops in Dera Ghazi Khan and Rajanpur, the two districts sandwiched between hill torrents from mountains in the west and river Indus in the east.
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Natural disasters, especially floods, can destroy roads. Elko County will repair and maintain those county roads that have been accepted as publically maintained roads. However, roads that have not been accepted for maintenance such as subdivision roads are the responsibility of the landowners who use those roads. A dry creek bed can become a raging torrent and wash out roads, bridges, and culverts. Residents served by private roads and/or bridges have been hit with large bills for repairs and/or reconstruction after floods.
Nevada is an open grazing law state. The cattle are free ranging. Fencing out of cattle is the responsibility of the property owner. Mother Nature The physical characteristics of your property can be positive and negative. Trees and other ground cover are a wonderful environmental amenity, but can also involve your home in a wild land fires, flooding, and other potential disasters. Below is a non comprehensive list of some of these issues. 2b1af7f3a8